The following is a list of the trophies from the Punch-Out!! series that appear in the 3DS. There are 6 trophies in total.
| Name | How to Unlock | Picture | Description |
| Little Mac | Classic Mode |  |
 This little boxer from the Punch-Out!! series makes up in heart what he lacks in height. He wasn't afraid to take on the boxing champs, and he's not afraid of the Nintendo stars. In Smash Bros., he packs a serious punch. When his KO Meter fills up, wade in and trigger a devastating uppercut. The little guy from Punch-Out!! has come a long way since his humble beginnings fighting the almost literal boxing giants. Above his damage display, you'll notice he has a little meter that fills up as he fights. When it's full, you'll see a flashing "KO" there. That means you can unleash a devastating uppercut!
|
| Little Mac (Alt.) | All-Star |  |
No matter his opponent, Little Mac always puts his best fist forward. He enters a charge state before unleashing his Straight Lunge special and can unleash it while charging by pressing the button again. He won't flinch while charging. At max charge, the launching power of this move is scary! Little Mac always puts his best fist forward, especially when you use Straight Lunge! You start by charging, then unleash the punch whenever you press the button again. Charge it to the max and its launching power is formidable! Also, attacks won't make you flinch while you're charging or once you've fully charged the move.
|
| Doc Louis | TBA |  |
Every great boxer needs a capable teacher. Little Mac lucked out with former famous heavyweight Doc Louis. If he's not eating his favorite chocolate bar or making jokes, he's sparring with Little Mac. He'll gladly take a knockout blow from Little Mac if it will help the boxer make it big! Behind every great boxer is a first-class coach, and energetic former heavy-weight champion Doc Louis is Little Mac's. Now and then, he'll put his chocolate bar down, his game face on, and spar with Little Mac, throwing punches just like in his glory days. No one's happier to take a knockout blow from Little Mac than Doc Louis!
|
| Glass Joe | TBA |  |
It's either very brave or very silly for a boxer to have a name so close to "glass jaw." When this French boxer takes a serious hit, he'll see croissants instead of stars. To his credit, Glass Joe never gives up—he's lost more bouts than most have fought in, and yet he never surrenders. Glass Joe might not sound exactly like "glass jaw", but you've got to admit, it takes guts to go into the ring with a name that tells everyone your weak point. Knock him out and...it'll rain croissants. Weird things aside, there's one thing you can say about Glass Joe: he's a man who doesn't give up, no matter how many times he loses.
|
| Bald Bull | TBA |  |
This 36-year-old Turkish boxer is in the prime of his career. He often tries to intimidate his opponent before a match even starts. He'll snort through his nose before charging his foe, a move called the Bull Charge. If he lands this blow, you can be sure Little Mac will hit the mat. A 36-year-old Turkish boxer in the prime of his career. His name hints at his bullish nature. In the match itself, you'll see him snort through his nostrils before using his special move, the Bull Charge. Little Mac's going to need to keep all his wits about him to avoid being bowled over by that one.
|
| Mr. Sandman | TBA |  |
This American boxer from Philadelphia is known for his speedy punches and brash style. He takes his name from a children's tale about a mythical character who brought sleep to people. When you face him in the ring, make sure you're the one sending him off to dreamland. This American boxer from Philadelphia is known for his speedy punches and brash, straightforward style. His ring name comes from a German folk tale about a fairy who makes people fall asleep. When you face him in the ring, make sure he's the one who boards the express train to dreamland.
|